イセキ　ヒロシ   Iseki Hiroshi   伊関　洋    所属   医学研究科 医学研究科 (医学部医学科をご参照ください)    職種   特任顧問
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Development of a Small-sized Leukapheresis Column for the Treatment of Inflammatory Diseases
掲載誌名	正式名：東京女子医科大学雑誌 ISSNコード：00409022
巻・号・頁	85(3),pp.93-101
著者・共著者	SHIMAGAKI Masaaki†, SUZUKI Takashi, ISEKI Hiroshi, MURAGAKI Yoshihiro
発行年月	2015/06
概要	Cytapheresis methods have been extended to remove cells such as leukocytes components, and are used for autoimmune disease treatment. However, the large blood volumes in conventional columns may restrict clinical use. We developed a small cytapheresis column. We evaluated its leukocyte adsorption property and inhibitory effect on gonarthritis. The priming volume was reduced to approximately one-third that of conventional columns. Leukocytes adsorbed to a mini-column for rabbits were counted following a 1-hour circulation immediately and 24 hours after the elicitation of inflammation. The diameter of joint swelling was measured at 24 hours and 48 hours. The adsorption property was found to differ; the number of removed leukocytes in the conventional column was largerimmediately after the elicitation, but was smaller at 24 hours, compared to the developed column. Thus, the developed column has a higher adsorption rate. For the inhibitory effect on gonarthritis, swelling decreased in the developed column group before and after the 24-hour circulation, compared with the sham column. Thus, the developed and conventional columns have equal inhibitory effects. These results show that the developed column improved selectivity and removal efficiency for granulocytes and monocytes and showed performance comparable to the conventional column.
NAID	110009915565