オカノ　テルオ   Okano Teruo   岡野　光夫    所属   医学研究科 医学研究科 (医学部医学科をご参照ください)    職種   評議員
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Cartilage repair in transplanted scaffold-free chondrocyte sheets using a minipig model.
掲載誌名	正式名：Biomaterials 略　 称：Biomaterials ISSNコード：1878-5905(Electronic)0142-9612(Linking)
巻・号・頁	33(15),pp.3846-51
著者・共著者	Ebihara Goro†, Sato Masato*, Yamato Masayuki, Mitani Genya, Kutsuna Toshiharu, Nagai Toshihiro, Ito Satoshi, Ukai Taku, Kobayashi Miyuki, Kokubo Mami, Okano Teruo, Mochida Joji
発行年月	2012/05
概要	Lacking a blood supply and having a low cellular density, articular cartilage has a minimal ability for self-repair. Therefore, wide-ranging cartilage damage rarely resolves spontaneously. Cartilage damage is typically treated by chondrocyte transplantation, mosaicplasty or microfracture. Recent advances in tissue engineering have prompted research on techniques to repair articular cartilage damage using a variety of transplanted cells. We studied the repair and regeneration of cartilage damage using layered chondrocyte sheets prepared on a temperature-responsive culture dish. We previously reported achieving robust tissue repair when covering only the surface layer with layered chondrocyte sheets when researching partial-thickness defects in the articular cartilage of domestic rabbits. The present study was an experiment on the repair and regeneration of articular cartilage in a minipig model of full-thickness defects. Good safranin-O staining and integration with surrounding tissues was achieved in animals transplanted with layered chondrocyte sheets. However, tissue having poor safranin-O staining-not noted in the domestic rabbit experiments-was identified in some of the animals, and the subchondral bone was poorly repaired in these. Thus, although layered chondrocyte sheets facilitate articular cartilage repair, further investigations into appropriate animal models and culture and transplant conditions are required.
DOI	10.1016/j.biomaterials.2012.01.056
文献番号	22369960