オカノ　テルオ   Okano Teruo   岡野　光夫    所属   医学研究科 医学研究科 (医学部医学科をご参照ください)    職種   評議員
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Repair of articular cartilage defect with layered chondrocyte sheets and cultured synovial cells.
掲載誌名	正式名：Biomaterials 略　 称：Biomaterials ISSNコード：1878-5905(Electronic)0142-9612(Linking)
巻・号・頁	33(21),pp.5278-86
著者・共著者	Ito Satoshi†, Sato Masato*, Yamato Masayuki, Mitani Genya, Kutsuna Toshiharu, Nagai Toshihiro, Ukai Taku, Kobayashi Miyuki, Kokubo Mami, Okano Teruo, Mochida Joji
発行年月	2012/07
概要	In this study, we investigate the effects of treatment with layered chondrocyte sheets and synovial cell transplantation. An osteochondral defect was created of 48 Japanese white rabbits. In order to determine the effects of treatment, the following 6 groups were produced: (A) synovial cells (1.8 × 10(6) cells), (B)layered chondrocyte sheets (1.7 × 10(6) cells), (C) synovial cells (3.0 × 10(5) cells) + layered chondrocyte sheets, (D)synovial cells (6.0 × 10(5) cells) + layered chondrocyte sheets, (E)synovial cells (1.2 × 10(6) cells) + layered chondrocyte sheets, (F) osteochondral defect. Layered chondrocyte sheets and synovial cells were transplanted, sacrificed four and 12 weeks postoperatively. An incapacitance tester (Linton) was used to find trends in the weight distribution ratio of the damaged limbs after surgery. Sections were stained with Safranin-O. Repair sites were evaluated using ICRS grading system. In groups (A) to (E), the damaged limb weight distribution ratio had improved. The repair tissue stained positively with Safranin-O. Four and 12 weeks after surgery, groups (A) to (E) exhibited significantly higher scores than group (F), and groups (D) and (E) exhibited significantly higher scores than groups (A) and (B). This suggests the efficacy of combining layered chondrocyte sheets with synovial cells.
DOI	10.1016/j.biomaterials.2012.03.073
文献番号	22551484