オカノ　テルオ   Okano Teruo   岡野　光夫    所属   医学研究科 医学研究科 (医学部医学科をご参照ください)    職種   評議員
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	In vivo cell tracking by bioluminescence imaging after transplantation of bioengineered cell sheets to the knee joint.
掲載誌名	正式名：Biomaterials 略　 称：Biomaterials ISSNコード：1878-5905(Electronic)0142-9612(Linking)
巻・号・頁	35(7),pp.2199-206
著者・共著者	Takaku Yuko†, Murai Kunihiko, Ukai Taku, Ito Satoshi, Kokubo Mami, Satoh Masaaki, Kobayashi Eiji, Yamato Masayuki, Okano Teruo, Takeuchi Mamoru, Mochida Joji, Sato Masato*
発行年月	2014/02
概要	In our previous studies, we have demonstrated effective regeneration of cartilage through the creation and application of layered cell sheets that combine both chondrocytes and synovial cells. In this study, we were able to demonstrate that cells derived from cell sheets can survive for long periods after transplantation into rat knee joints having osteochondral defects. We established a method for generating cell sheets from firefly luciferase-expressing chondrocytes obtained from transgenic Lewis rats, and carried out allogenic transplantation of these cell sheets into wild-type Lewis rats. We then administered luciferin and monitored the survival of the transplanted cells by using bioluminescence imaging (BLI). Our data showed that the transplanted cells survived and could be detected for more than 21 months, which was longer than expected. Furthermore, the BLI data showed that the transplanted cells remained in the knee joint and did not migrate to other parts of the body, thus confirming the safety of the cell sheets. In this study, we monitored the duration of survival of cell sheets composed of only chondrocytes, only synovial cells, or both chondrocytes and synovial cells, and found that all three types of cell sheets survived for an extended period of time.
DOI	10.1016/j.biomaterials.2013.11.071
文献番号	24360579