デジマ　カツフミ   Dejima Katsufumi   出嶋　克史    所属   医学部 医学科    職種   助教
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Syndecan defines precise spindle orientation by modulating Wnt signaling in C. elegans.
掲載誌名	正式名：Development (Cambridge, England) 略　 称：Development ISSNコード：1477-9129(Electronic)0950-1991(Linking)
巻・号・頁	141(22),pp.4354-65
著者・共著者	Dejima Katsufumi, Kang Sukryool, Mitani Shohei, Cosman Pamela C, Chisholm Andrew D
担当区分	筆頭著者
発行年月	2014/11
概要	Wnt signals orient mitotic spindles in development, but it remains unclear how Wnt signaling is spatially controlled to achieve precise spindle orientation. Here, we show that C. elegans syndecan (SDN-1) is required for precise orientation of a mitotic spindle in response to a Wnt cue. We find that SDN-1 is the predominant heparan sulfate (HS) proteoglycan in the early C. elegans embryo, and that loss of HS biosynthesis or of the SDN-1 core protein results in misorientation of the spindle of the ABar blastomere. The ABar and EMS spindles both reorient in response to Wnt signals, but only ABar spindle reorientation is dependent on a new cell contact and on HS and SDN-1. SDN-1 transiently accumulates on theABar surface as it contacts C, and is required for local concentration of Dishevelled (MIG-5) in the ABar cortex adjacent to C. These findings establish a new role for syndecan in Wnt-dependent spindle orientation.
DOI	10.1242/dev.113266
文献番号	25344071