タナベ　ケンジ   Tanabe Kenji   田邊　賢司    所属   研究施設 研究施設    職種   准教授
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Fission of tubular endosomes triggers endosomal acidification and movement.
掲載誌名	正式名：PLoS ONE
巻・号・頁	6,pp.e19764
著者・共著者	Mesaki, Kumi Tanabe, Kenji Obayashi, Masanori Oe, Natsuko Takei, Kohji
担当区分	筆頭著者,責任著者
発行年月	2011
概要	The early endosome acts as a sorting station for internalized molecules destined for recycling or degradation. While recycled molecules are sorted and delivered to tubular endosomes, residual compartments containing molecules to be degraded undergo"maturation"before final degradation in the lysosome. This maturation involves acidification, microtubule-dependent motility, and perinuclear localization. It is currently unknown how sorting and the processes of maturation cooperate with each other. Here, we show that fission of a tubular endosome triggers the maturation of the residual endosome, leading to degradation. Use of the dynamin inhibitor dynasore to block tubular endosome fission inhibited acidification, endosomal motility along microtubules, perinuclear localization, and degradation. However, tubular endosome fission was not affected by inhibiting endosomal acidification or by depolymerizing the microtubules. These results demonstrate that the fission of recycling tubules is the first important step in endosomal maturation and degradation in the lysosome. We believe this to be the first evidence of a cascade from sorting to degradation.