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Kyohei Fujita
Department Research Institutes and Facilities, Research Institutes and Facilities Position Assistant Professor (Fixed Term) |
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| Language | English |
| Title | Characterizing and Modulating the Mechanical Properties of hydrogels from Ventricular Extracellular Matrix |
| Conference | 10th Asian Control Conference 2015 |
| Conference Type | International society and overseas society |
| Presentation Type | Speech |
| Lecture Type | General |
| Publisher and common publisher | ◎Fujita Kyohei, Tsuchida Yuuki, Seki Hiroshi, Sato Daisuke, Nakumura Takao, Kosawada Tadashi, Feng Zhonggang, Shiraishi Yasuyuki, Umezu Mitsuo |
| Date | 2015/06/01 |
| Venue (city and name of the country) |
Kota Kinabalu, Malaysia |
| Holding period | 2015/05/31～2015/06/03 |
| Society abstract | Proceedings of the 10th Asian Control Conference 2015 718-722 2015 |
| Summary | In order to differentiate pluripotent stem cells to cardiomyocytes, the most general method is to expose stem cells to various growth factors related to cardiogenesis. However, a novel method has been reported to induce cardiac differentiation of human ES cells without supplemental growth factors by culturing embryoid body of human ES cells in hybrid gels composed of cardiac extracellular matrix (ECM) and type I collagen. On the other hand, mechanical properties of scaffold is one of the critical cue for differentiation of stem cells. However, it has not been thoroughly investigated the mechanical properties of the scaffold made from cardiac ECM in view of this and other reports about the differentiation of stem cells into cardiomyocytes using cardiac ECM scaffold. In this study, we fabricated bio-hydrogels composed of goat ventricular extracellular matrix, and investigated the mechanical properties by means of uniaxial compression test. It showed that the ECM gels possess viscoelastic property. The elastic modulus K 1 in modified non-linear Kelvin model is 9.5 Pa for these gels and K 2 is 814.7 Pa. Moreover, we were able to improve the elastic moduli K 1 and K 2 up to 139.7 Pa and 2023.9 Pa, respectively, by chemical treatment using EDAC. |