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Maru Yoshiro
Department School of Medicine, School of Medicine Position Professor and Division head |
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| Article types | Original article |
| Language | English |
| Peer review | Peer reviewed |
| Title | ZFC3H1, a zinc finger protein, modulates IL-8 transcription by binding with celastramycin A, a potential immune suppressor. |
| Journal | Formal name：PloS one Abbreviation：PLoS One ISSN code：(1932-6203)1932-6203(Linking) |
| Volume, Issue, Page | 9(9),pp.e108957 |
| Author and coauthor | Tomita Takeshi*, Ieguchi Katsuaki, Coin Fredric, Kato Yasuhiro, Kikuchi Haruhisa, Oshima Yoshiteru, Kurata Shoichiro, Maru Yoshiro* |
| Authorship | Last author,Corresponding author |
| Publication date | 2014 |
| Summary | Celastramycin A, a small molecule that inhibits the production of antibacterial peptides in an ex vivo culture system of Drosophila, suppresses the TNFα-mediated induction of IL-8 in mammalian cells. To understand its molecular mechanism, we examined Celastramycin A binding proteins and investigated their biological functions. Our screening and subsequent pull-down assay revealed ZFC3H1 (also known as CCDC131 or CSRC2), an uncharacterized zinc finger protein, as a Celastramycin A binding protein. The knockdown of ZFC3H1 reduced IL-8 expression levels in the TNFα-stimulated lung carcinoma cell line, LU99, and UV-irradiated HeLa cells. Based on reporter assay results, we concluded that ZFC3H1 participates in the transcriptional activation of IL-8. The findings of our UV-irradiation experiments implied that ZFC3H1 may indirectly interact with ERCC1 in an activated DNA repair complex. Thus, we designated ZFC3H1 as a mammalian target of Celastramycin A (mTOC). |
| DOI | 10.1371/journal.pone.0108957 |
| PMID | 25268596 |