マル　ヨシロウ   Maru Yoshiro   丸　義朗    所属   医学部 医学科    職種   教授・基幹分野長
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	ZFC3H1, a zinc finger protein, modulates IL-8 transcription by binding with celastramycin A, a potential immune suppressor.
掲載誌名	正式名：PloS one 略　 称：PLoS One ISSNコード：(1932-6203)1932-6203(Linking)
巻・号・頁	9(9),pp.e108957
著者・共著者	Tomita Takeshi*, Ieguchi Katsuaki, Coin Fredric, Kato Yasuhiro, Kikuchi Haruhisa, Oshima Yoshiteru, Kurata Shoichiro, Maru Yoshiro*
担当区分	最終著者,責任著者
発行年月	2014
概要	Celastramycin A, a small molecule that inhibits the production of antibacterial peptides in an ex vivo culture system of Drosophila, suppresses the TNFα-mediated induction of IL-8 in mammalian cells. To understand its molecular mechanism, we examined Celastramycin A binding proteins and investigated their biological functions. Our screening and subsequent pull-down assay revealed ZFC3H1 (also known as CCDC131 or CSRC2), an uncharacterized zinc finger protein, as a Celastramycin A binding protein. The knockdown of ZFC3H1 reduced IL-8 expression levels in the TNFα-stimulated lung carcinoma cell line, LU99, and UV-irradiated HeLa cells. Based on reporter assay results, we concluded that ZFC3H1 participates in the transcriptional activation of IL-8. The findings of our UV-irradiation experiments implied that ZFC3H1 may indirectly interact with ERCC1 in an activated DNA repair complex. Thus, we designated ZFC3H1 as a mammalian target of Celastramycin A (mTOC).
DOI	10.1371/journal.pone.0108957
PMID	25268596