カンノ　ヒトシ   菅野　仁    所属   医学部 医学科（東京女子医科大学病院）    職種   特任教授
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Upregulation of tumor necrosis factor receptor 1 and TNF-alpha converting enzyme during corneal wound healing.
掲載誌名	正式名：Japanese journal of ophthalmology 略　 称：Jpn J Ophthalmol ISSNコード：0021-5155(Print)0021-5155(Linking)
巻・号・頁	52(5),pp.393-8
著者・共著者	Sakimoto Tohru, Yamada Ai, Kanno Hitoshi, Sawa Mitsuru
発行年月	2011/12
概要	PURPOSE:To elucidate tumor necrosis factor (TNF) receptor-1 (TNFR1) and TNF-alpha converting enzyme (TACE) involvement during corneal wound healing.METHODS:The corneas of BALB/c mice cornea were scarred by alkali burns using filter paper dipped in 1N NaOH solution. TACE and TNFR1 expression in the alkali-burned corneas were evaluated by immunohistochemistry (total, 16 eyes). Using cultured fibroblasts (human) and macrophages (mice), we evaluated the release of soluble TNF-alpha (sTNF-alpha) and soluble TNFR1 (sTNFR1) in the supernatant by enzyme-linked immunosorbent assay after stimulating TACE activity with phorbol myristate acetate (PMA).RESULTS:In alkali-burned corneas, both TACE and TNFR1 expression were observed in the stromal cells after the acute phase of wound healing response. In macrophage-cultured supernatant, both sTNF-alpha and sTNFR1 release were promoted by PMA stimulation. On the other hand, only sTNFR1 released by PMA stimulation was observed in fibroblast-cultured supernatant.CONCLUSIONS:TACE and TNFR1 were expressed mainly after the acute phase of corneal wound healing. The TACE-dependent extracellular release of sTNFR1 was recognized in cultured fibroblasts and macrCONCLUSIONS:ophages.
DOI	10.1007/s10384-008-0536-8
文献番号	18991041