Kato Hidehito    Department   Center for Medical and Nursing Education, Center for Medical and Nursing Education    Position
Article types	Original article
Language	English
Peer review	Peer reviewed
Title	High affinity of interaction between superantigen and TCR Vβ molecules induces a high level and prolonged expansion of superantigen-reactive CD4+ T cells.
Journal	Formal name：The Journal of biological chemistry Abbreviation：J Biol Chem ISSN code：00219258/1083351X
Volume, Issue, Page	285(40),pp.30427-30435
Author and coauthor	OMOE Katsuhiko† ,NUNOMURA Wataru ,KATO Hidehito ,LI Zhong-Juan ,IGARASHI Osamu ,ARAAKE Minako ,SANO Keigo ,ONO Hisaya K ,ABE Yohei ,HU Dong-Liang ,NAKANE Akio ,KIYONO Hiroshi ,TAKAKUWA Yuichi ,SHINAGAWA Kunihiro ,UCHIYAMA Takehiko ,IMANISHI Ken'ichi*
Publication date	2010/10
Summary	In mice implanted with an osmotic pump filled with the superantigen (SAG) staphylococcal enterotoxin A (SEA), the Vbeta3+CD4+ T cells exhibited a high level of expansion whereas the Vbeta11+CD4+ T cells exhibited a mild level of expansion. In contrast, in mice implanted with an osmotic pump filled with SE-like type P (SElP, 78.1% homologous with SEA), the Vbeta11+CD4+ T cells exhibited a high level of expansion while the Vbeta3+CD4+ T cells exhibited a low level of expansion, suggesting that the level of the SAG-induced response is determined by the affinities between the TCR Vbeta molecules and SAG. Analyses using several hybrids of SEA and SElP showed that residue 206 of SEA determines the response levels of Vbeta3+CD4+ and Vbeta11+CD4+ T cells both in vitro and in vivo. Analyses using the above-mentioned hybrids showed that the binding affinities between SEA and the Vbeta3/Vbeta11 beta chains and between SEA-MHC class II-molecule complex and Vbeta3+/Vbeta11+ CD4+ T cells determines the response levels of the SAG-reactive T cells both in vitro and in vivo.