ミタニ シヨウヘイ
MITANI Shohei
三谷 昌平 所属 医学部 医学科 職種 教授・基幹分野長 |
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論文種別 | 原著 |
言語種別 | 英語 |
査読の有無 | 査読あり |
表題 | GOP-1 promotes apoptotic cell degradation by activating the small GTPase Rab2 in C. elegans. |
掲載誌名 | 正式名:The Journal of cell biology 略 称:J Cell Biol ISSNコード:(1540-8140)0021-9525(Linking) |
掲載区分 | 国外 |
巻・号・頁 | 216(6),pp.1775-1794 |
著者・共著者 | Yin Jianhua, Huang Yaling, Guo Pengfei, Hu Siqi, Yoshina Sawako, Xuan Nan, Gan Qiwen, Mitani Shohei, Yang Chonglin, Wang Xiaochen |
発行年月 | 2017/06 |
概要 | Apoptotic cells generated by programmed cell death are engulfed by phagocytes and enclosed within plasma membrane-derived phagosomes. Maturation of phagosomes involves a series of membrane-remodeling events that are governed by the sequential actions of Rab GTPases and lead to formation of phagolysosomes, where cell corpses are degraded. Here we identified gop-1 as a novel regulator of apoptotic cell clearance in Caenorhabditis elegans Loss of gop-1 affects phagosome maturation through the RAB-5-positive stage, causing defects in phagosome acidification and phagolysosome formation, phenotypes identical to and unaffected by loss of unc-108, the C. elegans Rab2 GOP-1 transiently associates with cell corpse-containing phagosomes, and loss of its function abrogates phagosomal association of UNC-108. GOP-1 interacts with GDP-bound and nucleotide-free UNC-108/Rab2, disrupts GDI-UNC-108 complexes, and promotes activation and membrane recruitment of UNC-108/Rab2 in vitro. Loss of gop-1 also abolishes association of UNC-108 with endosomes, causing defects in endosome and dense core vesicle maturation. Thus, GOP-1 is an activator of UNC-108/Rab2 in multiple processes. |
DOI | 10.1083/jcb.201610001 |
PMID | 28424218 |