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Kikuchi Ken
Department School of Medicine(Tokyo Women's Medical University Hospital), School of Medicine Position Professor |
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| Article types | Original article |
| Language | English |
| Peer review | Peer reviewed |
| Title | Development of an appropriate PCR system for the reclassification of Streptococcus suis. |
| Journal | Formal name：Journal of microbiological methods Abbreviation：J Microbiol Methods ISSN code：(1872-8359)0167-7012(Linking) |
| Domestic / Foregin | Foregin |
| Volume, Issue, Page | 107,pp.66-70 |
| Author and coauthor | Ishida Sakura, Tien Le Hong Thuy, Osawa Ro, Tohya Mari, Nomoto Ryohei, Kawamura Yoshiaki, Takahashi Tatsuhumi, Kikuchi Naoya, Kikuchi Ken, Sekizaki Tsutomu |
| Publication date | 2014/12 |
| Summary | Thirty-five serotypes of Streptococcus suis (serotypes 1-34 and serotype 1/2) have so far been described on the basis of their polysaccharide capsular antigens. However, in the last decade, some serotype reference strains have been reexamined for their taxonomic status, and the reference strains of serotypes 20, 22, 26, 32, 33, and 34 may be different from taxon S. suis. In the present study, we developed a novel PCR method targeting the recombination/repair protein (recN) gene of S. suis, designated recN PCR, which corresponds to the current reclassification of this bacterium. We compared its specificity with other PCR methods for S. suis, and the results obtained confirmed its specificity. In addition, the detection limits of recN PCR were similar among all the reference strains of authentic S. suis, indicating that the recN PCR gave reliable results against bacterial strains and isolates used in this study. Therefore, recN PCR described in the present study will be a useful tool for the identification of authentic S. suis, and can also be used in epidemiological studies on this bacterium. |
| DOI | 10.1016/j.mimet.2014.09.003 |
| PMID | 25229648 |