ノハラ　ミチコ   NOHARA Michiko   野原　理子    所属   統合教育学修センター 統合教育学修センター    職種   教授
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Augmentation of allergic inflammation in the airways of cyclooxygenase-2-deficient mice.
掲載誌名	正式名：Respirology (Carlton, Vic.) 略　 称：Respirology ISSNコード：1323779913237799
掲載区分	国外
巻・号・頁	10(2),pp.149-156
著者・共著者	Nakata Junko, Kondo Mitsuko, Tamaoki Jun, Takemiya Takako, Nohara Michiko, Yamagata Kanato, Nagai Atsushi
発行年月	2005/03
概要	OBJECTIVE:Airway cyclooxygenase-2 (COX-2) is induced by cytokine-mediated inflammation such as occurs in asthma. However, the role of COX-2 in the pathophysiology of asthma is not fully understood.METHODS:Allergic inflammation, airway responsiveness to methacholine and mucous cell metaplasia after ovalbumin sensitization in the airways of COX-2 deficient (-/-) mice, COX-2 (+/+) mice and C57BL/6J mice treated with a selective COX-2 inhibitor, nimesulide were assessed. Histology, cell analysis, measurements of arachidonic acid metabolites and Th2 cytokine levels in bronchoalveolar lavage fluid (BALF), and measurement of serum IgE were performed.RESULTS:Eosinophil infiltration into the airway wall, and the number of eosinophils in BALF were greater in sensitized COX-2 (-/-) mice than in sensitized COX-2 (+/+) mice. The levels of cysteinyl leukotrienes (LTC4/D4/E4), prostaglandin E2 (PGE2) and interleukin (IL)-13 as well as airway responsiveness did not differ in COX-2 (-/-) mice and COX-2 (+/+) mice. However, sensitized COX-2 (-/-) mice had higher LTC4/D4/E4 and lower PGE2 concentrations compared with non-sensitized COX-2 (-/-) mice. The number of PAS/alcian blue-positive airway epithelial cells and serum IgE were elevated in COX-2 (-/-) mice. Nimesulide-treated mice showed augmented eosinophilic inflammation, LTC4/D4/E4 concentrations and mucous cell metaplasia.CONCLUSION:These data indicate that COX-2 deficiency augments allergic inflammation and mucous cell metaplasia.
DOI	10.1111/j.1440-1843.2005.00687.x
PMID	15823178