MARUYAMA Takashi    Department   School of Medicine(Tokyo Women's Medical University Hospital), School of Medicine    Position
Article types	Original article
Language	Japanese
Peer review	Peer reviewed
Title	Methylation- and Transcription-based Clonality Analysis of Oligodendroglioma
Domestic / Foregin	Domestic
Volume, Issue, Page	pp.251-259
Authorship	Lead author
Publication date	1999/05
Summary	Characterization of the clonal derivation of human neoplasms has provided important information about the etiology and pathogenesis, and has oractical implications for both the diagnosis and subsequent studies on disease progression. ln the oresent study, molecular srenetic assays, including methylation- and transcription-based donal techniques were used to determine the clonality of 13 oligodendroglioma oatients (16 samples). Analysis of the tumors with PCR-based methylation assays at the androgen receotor locus (HUMARA)confirmed that three informative benign sarrmles and two of eight malignant sairmles were monoclonal, and the remaining six malignant samples were all polyclonal. Of three patients who had two separate operations, two consistently showed both monoclonal, and one showed monoclonal at the first operation, then changed to polyclonal. Transcription-based analysis showed four informative benign samples and three of six were malignant samples were monoclonal, while the remaining three were polyclonal. Two samples, which showed polyclonal findings by methylation-based analysis, were demonstrated to be monoclonal by transcription-based analysis. Our data demonstrated that many tumors consist of homogeneous clonal cells in origin but some are mixed, showing both heterogeneous cells and other populations of clonal cells.
Document No.	1999246717
PermalinkURL	http://hdl.handle.net/10470/24726