YAMATO Masayuki    Department   Research Institutes and Facilities, Research Institutes and Facilities    Position   Professor
Article types	Original article
Language	English
Peer review	Peer reviewed
Title	Type I collagen-induced YAP nuclear expression promotes primary cilia growth and contributes to cell migration in confluent mouse embryo fibroblast 3T3-L1 cells.
Journal	Formal name：Molecular and cellular biochemistry Abbreviation：Mol Cell Biochem ISSN code：(1573-4919)0300-8177(Linking)
Domestic / Foregin	Foregin
Publisher	Springer
Volume, Issue, Page	450(1-2),pp.87-96
Author and coauthor	XU Qian†, LIU Xiaoling, LIU Weiwei, HAYASHI Toshihiko, YAMATO Masayuki, FUJISAKI Hitomi, HATTORI Shunji, TASHIRO Shin-Ichi, ONODERA Satoshi, IKEJIMA Takashi*
Publication date	2019/01
Summary	The extracellular matrix (ECM) is a major biomechanical environment for all cells in vivo, and tightly controls wound healing and cancer progression. Type I collagen (Col I) is the most abundant component in ECM and plays an essential role for cell motility control and migration beyond structural support. Our previous results showed that Col I increased the length of primary cilia and the expression of primary cilia-associated proteins in 3T3-L1 cells. The Hippo/YAP pathway serves as a major integrator of cell surface-mediated signals and regulates key processes for the development and maintenance of tissue functions. In this study, we investigated the role of Hippo/YAP signaling in primary cilia growth of cells cultured on Col I-coated plate, as well as the potential link between primary cilia and migration. At 2-day post-confluence, YAP localization in the nucleus was dramatically increased when the cells were cultured on Col I-coated plate, accompanied by cilia growth. YAP inhibitor verteporfin repressed the growth of primary cilia as well as the expressions of ciliogenesis-associated proteins in confluent 3T3-L1 cells cultured on Col I-coated plate. Moreover, knockdown of either YAP or IFT88, one of the ciliogenesis-associated proteins, reversed the migration of confluent 3T3-L1 cells promoted by Col I-coating. In conclusion, activation of YAP pathway by Col I-coating of culture plate for confluent 3T3-L1 cells is positively associated with the primary cilia growth, which eventually results in promoted migration.
DOI	10.1007/s11010-018-3375-z
PMID	29846859