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ヤマト マサユキ
YAMATO Masayuki
大和 雅之 所属 研究施設 研究施設 職種 教授 |
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| 論文種別 | 原著 |
| 言語種別 | 英語 |
| 査読の有無 | 査読あり |
| 表題 | Type I collagen induces mesenchymal cell differentiation into myofibroblasts through YAP-induced TGF-β1 activation. |
| 掲載誌名 | 正式名：Biochimie 略 称：Biochimie ISSNコード：(1638-6183)0300-9084(Linking) |
| 掲載区分 | 国外 |
| 出版社 | Elsevier |
| 巻・号・頁 | 150,pp.110-130 |
| 著者・共著者 | LIU Xiaoling†, LONG Xinyu, LIU Weiwei, ZHAO Yeli, HAYASHI Toshihiko, YAMATO Masayuki, MIZUNO Kazunori, FUJISAKI Hitomi, HATTORI Shunji, TASHIRO Shin-Ichi, OGURA Takaaki, ATSUZAWA Yuji, IKEJIMA Takashi* |
| 発行年月 | 2018/07 |
| 概要 | In organ fibrosis, mechanical stress and transforming growth factor beta-1 (TGF-β1) promote differentiation into myofibroblast from mesenchymal cells, leading to extracellular matrix (ECM) remodeling or active synthesis, deposition or degradation of ECM components. A major component of ECM, type I collagen (col I) triple helical molecules assemble into fibrils or are denatured to gelatin without triple-helicity in remodeling. However, whether changes of ECM components in remodeling have influence on mesenchymal cell differentiation remains elusive. This study adopted three states of collagen I existing in ECM remodeling: molecular collagen, fibrillar collagen and gelatin to see what are characteristics in the effects on two cell lines of mesenchymal origin, murine 3T3-L1 embryonic fibroblast and murine C2C12 myoblasts. The results showed that all three forms of collagen I were capable of inducing these two cells to differentiate into myofibroblasts characterized by increased expression of alpha-smooth muscle actin (α-SMA) mRNA. The expression of α-SMA is positively regulated by TGF-β1. Nuclear translocation of Yes-associated protein (YAP) is involved in this process. Focal adhesion kinase (FAK) is activated in the cells cultured on molecular collagen-coated plates, contributing to YAP activation. On the other hand, in the cells cultured on fibrillar collagen gel or gelatin-coated plates, oxidative stress but not FAK induce YAP activation. In conclusion, the three physicochemically distinct forms of col I induce the differentiation of mesenchymal cells into myofibroblasts through different pathways. |
| DOI | 10.1016/j.biochi.2018.05.005 |
| PMID | 29777737 |