YAMATO Masayuki
   Department   Research Institutes and Facilities, Research Institutes and Facilities
   Position   Professor
Article types Original article
Language English
Peer review Peer reviewed
Title Enhanced Migration of murin fibroblast-like 3T3-L1 preadipocytes on type I collagen-coated dish is reversed by silibinin treatment.
Journal Formal name：Molecular and cellular biochemistry
Abbreviation：Mol Cell Biochem
ISSN code：(1573-4919)0300-8177(Linking)
Domestic / ForeginForegin
Publisher Springer
Volume, Issue, Page 441(1-2),pp.35-62
Author and coauthor LIU Xiaoling†, XU Qian, YAO Guodong, YELI Zhao, XU Fanxing, HAYASHI Toshihiko, FUJISAKI Hitomi, HATTORI Shunji, TASHIRO Shin-ichi, ONODERA Satoshi, YAMATO Masayuki, IKEJIMA Takashi*
Publication date 2018/04
Summary Migration of fibroblast-like preadipocytes is important for the development of adipose tissue, whereas excessive migration is often responsible for impaired adipose tissue related with obesity and fibrotic diseases. Type I collagen (collagen I) is the most abundant component of extracellular matrix and has been shown to regulate fibroblast migration in vitro, but its role in adipose tissue is not known. Silibinin is a bioactive natural flavonoid with antioxidant and antimetastasis activities. In this study, we found that type I collagen coating promoted the proliferation and migration of murine 3T3-L1 preadipocytes in a dose-dependent manner, implying that collagen I could be an extracellular signal. Regarding the mechanisms of collagen I-stimulated 3T3-L1 migration, we found that NF-κB p65 is activated, including the increased nuclear translocation of NF-κB p65 as well as the upregulation of NF-κB p65 phosphorylation and acetylation, accompanied by the increased expressions of proinflammatory factors and the generation of reactive oxygen species (ROS). Reduction of collagen I-enhanced migration of cells by treatment with silibinin was associated with suppression of NF-κB p65 activity and ROS generation, and negatively correlated with the increasing sirt1 expression. Taken together, the enhanced migration of 3T3-L1 cells induced on collagen I-coated dish is mediated by the activation of NF-κB p65 function and ROS generation that can be alleviated with silibinin by upregulation of sirt1, leading to the repression of NF-κB p65 function and ROS generation.
DOI 10.1007/s11010-017-3173-z
PMID 28933025