|
シミズ タツヤ
SHIMIZU Tatsuya
清水 達也 所属 研究施設 研究施設 職種 教授 |
|
| 論文種別 | 原著 |
| 言語種別 | 英語 |
| 査読の有無 | 査読あり |
| 表題 | Creation of myocardial tubes using cardiomyocyte sheets and an in vitro cell sheet-wrapping device. |
| 掲載誌名 | 正式名：Biomaterials 略 称：Biomaterials ISSNコード：(0142-9612)0142-9612(Linking) |
| 掲載区分 | 国外 |
| 出版社 | Elsevier |
| 巻・号・頁 | 28(24),pp.3508-16 |
| 著者・共著者 | KUBO Hirotsugu†, SHIMIZU Tatsuya, YAMATO Masayuki, FUJIMOTO Tetsuo, OKANO Teruo* |
| 担当区分 | 2nd著者 |
| 発行年月 | 2007/08 |
| 概要 | Regenerative medicine involving injection of isolated cells and transplantation of tissue-engineered myocardial patches, has received significant attention as an alternative method to repair damaged heart muscle. In the present study, as the next generation of myocardial tissue engineering we demonstrate the in vitro fabrication of pulsatile myocardial tubes using cell sheet engineering technologies. Three neonatal rat cardiomyocyte sheets, which were harvested from temperature-responsive culture dishes, were wrapped around fibrin tubes using a novel cell sheet-wrapping device. The tubular constructs demonstrated spontaneous, synchronized pulsation within 3h after cell sheet wrapping. Contractile force measurements showed that the contractile force increased in accordance with both increasing rest length (Starling mechanism) and increasing extracellular Ca(2+) concentration. Furthermore, the tissue-engineered myocardial tubes presented measurable inner pressure changes evoked by tube contraction (0.11+/-0.01mmHg, max 0.15mmHg, n=5). Histological analyses revealed both well-differentiated sarcomeres and diffuse gap junctions within the myocardial tissues that resembled native cardiac muscle. These data indicate that tissue-engineered myocardial tubes have native heart-like structure and function. These new myocardial tissue constructs should be useful for future applications in physiological studies and pharmacological screening, and present a possible core technology for the creation of engineered tissues capable of independent cardiac assistance. |
| DOI | 10.1016/j.biomaterials.2007.04.016 |
| PMID | 17482255 |