イナイ　シユンタ   稲井　俊太    所属   医学部 医学科（東京女子医科大学病院）    職種   助教
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Induction of apoptosis in the vestibule of guinea pigs after middle ear inoculation with lipopolysaccharide (LPS).
掲載誌名	正式名：Auris, nasus, larynx 略　 称：Auris Nasus Larynx ISSNコード：03858146/03858146
掲載区分	国外
巻・号・頁	30(4),pp.355-361
著者・共著者	Watanabe Ken-ichi†, Jinnouchi Ken, Inai Shunta, Yagi Toshiaki
発行年月	2003/12
概要	OBJECTIVE:During the process of apoptosis, double-stranded DNA is broken into single-stranded DNA (ssDNA) by the action of caspases and caspase activated deoxyribonuclease (CAD). We immunohistochemically examined the apoptotic changes induced by endotoxin in the vestibule of guinea pigs.METHODS:Lipopolysaccharide (LPS) (5 mg/ml, 0.2 ml), a bacterial endotoxin, was transtympanically injected into the middle ear. 48 h after injection of LPS, animals were sacrificed by intracardiac perfusion of fixative. The temporal bones were then removed and immunohistochemically stained for ssDNA, CAD and caspase 3.RESULTS:ssDNA was detected after 48 h in the dark cell area of the LPS group, not in the sensory epithelium. CAD was observed both in the dark cell area and the sensory epithelium. Caspase 3 was also detected both in the dark cell area and the sensory epithelium.CONCLUSION:Our findings suggest that apoptosis is involved in the dysfunction of the vestibule under inflammatory conditions.
DOI	10.1016/s0385-8146(03)00061-0
PMID	14656560