ササキ　ダイスケ   SASAKI Daisuke   佐々木　大輔    所属   研究施設 研究施設    職種   特任助教
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Elementary steps of the cross-bridge cycle in bovine myocardium with and without regulatory proteins.
掲載誌名	正式名：Biophysical journal 略　 称：Biophys J ISSNコード：00063495/00063495
掲載区分	国外
巻・号・頁	82(2),pp.915-28
著者・共著者	Fujita Hideaki, Sasaki Daisuke, Ishiwata Shin'ichi, Kawai Masataka
発行年月	2002/02
概要	The role of regulatory proteins in the elementary steps of the cross-bridge cycle in bovine myocardium was investigated. The thin filament was selectively removed by gelsolin and the actin filament was reconstituted without tropomyosin or troponin. Further reconstitution was achieved by adding tropomyosin and troponin. The effects of MgATP and phosphate (Pi) on the rate constants of exponential processes were studied in control, actin filament-reconstituted, and thin filament-reconstituted myocardium at pCa < or = 4.66, pH 7.00, 25 degrees C. In control myocardium, the MgATP association constant was 9.1 +/- 1.3 mM(-1), and the Pi association constant 0.14 +/- 0.04 mM(-1). The equilibrium constant of the cross-bridge detachment step was 2.6 +/- 0.4, and the equilibrium constant of the force generation step was 0.59 +/- 0.04. In actin filament-reconstituted myocardium without regulatory proteins, the MgATP association constant was approximately the same, and the Pi association constant increased to 2.8x. The equilibrium constant of cross-bridge detachment decreased to 0.2x, but the equilibrium constant of the force generation step increased to 4x. These kinetic constants regained control values after reconstitution of the thin filament. These results indicate that tension/cross-bridge in the presence of regulatory proteins is approximately 1.5-1.7x of that in the absence of regulatory proteins. These results further indicate that regulatory proteins promote detachment of cross-bridges.
DOI	10.1016/S0006-3495(02)75453-2
PMID	11806933