デジマ　カツフミ   Dejima Katsufumi   出嶋　克史    所属   医学部 医学科    職種   助教
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Distinct pathways for export of silencing RNA in Caenorhabditis elegans systemic RNAi.
掲載誌名	正式名：iScience 略　 称：iScience ISSNコード：25890042/25890042
掲載区分	国外
巻・号・頁	26(10),pp.108067
著者・共著者	YOSHIDA Keita†, SUEHIRO Yuji, DEJIMA Katsufumi, YOSHINA Sawako, MITANI Shohei*
発行年月	2023/10
概要	Dietary supplied double-stranded RNA (dsRNA) can trigger RNA interference (RNAi) systemically in some animals, including the nematode Caenorhabditis elegans. Although this phenomenon has been utilized as a major tool for gene silencing in C. elegans, how cells spread the silencing RNA throughout the organism is largely unknown. Here, we identify two novel systemic RNAi-related factors, REXD-1 and TBC-3, and show that these two factors together with SID-5 act redundantly to promote systemic spreading of dsRNA. Animals that are defective in all REXD-1, TBC-3, and SID-5 functions show strong deficiency in export of dsRNA from intestinal cells, whereas cellular uptake and processing of dsRNA and general secretion events other than dsRNA secretion are still functional in the triple mutant animals. Our findings reveal pathways that specifically regulate the export of dsRNA in parallel, implying the importance of spreading RNA molecules for intercellular communication in organisms.
DOI	10.1016/j.isci.2023.108067
PMID	37854694