ミヤヤマ　タカミツ   Takamitsu Miyayama   宮山　貴光    所属   医学部 医学科    職種   助教
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Copper accumulation and compartmentalization in mouse fibroblast lacking metallothionein and copper chaperone, Atox1
掲載誌名	正式名：Toxicology and Applied Pharmacology 略　 称：Toxicol Appl Pharmacol ISSNコード：10960333/0041008X
巻・号・頁	237(2),pp.205-13
著者・共著者	MIYAYAMA Takamitsu†, SUZUKI Kazuo, OGRA Yasumitsu*
担当区分	筆頭著者
発行年月	2009/06
概要	Copper (Cu) is the active center of some enzymes because of its redox-active property, although that property could have harmful effects. Because of this, cells have strict regulation/detoxification systems for this metal. In this study, multi-disciplinary approaches, such as speciation and elemental imaging of Cu, were applied to reveal the detoxification mechanisms for Cu in cells bearing a defect in Cu-regulating genes. Although Cu concentration in metallothionein (MT)-knockout cells was increased by the knockdown of the Cu chaperone, Atox1, the concentrations of the Cu influx pump, Ctr1, and another Cu chaperone, Ccs, were paradoxically increased; namely, the cells responded to the Cu deficiency despite the fact that cellular Cu concentration was actually increased. Cu imaging showed that the elevated Cu was compartmentalized in cytoplasmic vesicles. Together, the results point to the novel roles of MT and cytoplasmic vesicles in the detoxification of Cu in mammalian cells.
DOI	10.1016/j.taap.2009.03.024S0041-008X(09)00140-9 [pii]
文献番号	19362104