ヨシダ　ケイタ   YOSHIDA Keita   吉田　慶太    所属   医学部 医学科    職種   助教
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Germ cell mutations of the ascidian Ciona intestinalis with TALE nucleases.
掲載誌名	正式名：Genesis (New York, N.Y. : 2000) 略　 称：Genesis ISSNコード：1526-968X(Electronic)1526-954X(Linking)
掲載区分	国外
巻・号・頁	52(5),pp.431-9
著者・共著者	Yoshida Keita†, Treen Nicholas, Hozumi Akiko, Sakuma Tetsushi, Yamamoto Takashi, Sasakura Yasunori*
担当区分	筆頭著者
発行年月	2014/05
概要	Targeted mutagenesis of genes-of-interest, or gene-knockout, is a powerful method to address the functions of genes. Engineered nucleases have enabled this approach in various organisms because of their ease of use. The ascidian Ciona intestinalis is an excellent organism to analyze gene functions by means of genetic technologies. In our previous study, we reported mutagenesis of Ciona somatic cells with TALE nucleases (TALENs) by electroporating expression constructs. Inthis study, we report germ cell mutagenesis of Ciona by microinjecting mRNAs encoding TALENs. TALEN mRNAs introduced mutations to target genes in both somatic and germ cells. TALEN-mediated mutations in the germ cell genome were inherited by the next generation. We conclude that knockout lines of Ciona that have disrupted target genes can be established through TALEN-mediated germ cell mutagenesis.
DOI	10.1002/dvg.22770
文献番号	24619765