ヨシダ　ケイタ   YOSHIDA Keita   吉田　慶太    所属   医学部 医学科    職種   助教
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Chondroitin 6-O-sulfotransferases are required for morphogenesis of the notochord in the ascidian embryo.
掲載誌名	正式名：Developmental dynamics : an official publication of the American Association of Anatomists 略　 称：Dev Dyn ISSNコード：1097-0177(Electronic)1058-8388(Linking)
掲載区分	国外
巻・号・頁	243(12),pp.1637-45
著者・共著者	Nakamura Jun, Yoshida Keita, Sasakura Yasunori, Fujiwara Shigeki
担当区分	2nd著者
発行年月	2014/12
概要	BACKGROUND:Chondroitin sulfate (CS) is a sulfated polysaccharide chain that binds to various core proteins to form proteoglycans. The amount and position of sulfate groups in CS are variable among different tissues, and are determined by specific sulfotransferases. Although the ascidians are the closest relatives of vertebrates, the functions of their sulfotransferases have not been studied.RESULTS:The genome of the ascidian Ciona intestinalis contains eight genes encoding proteins similar to chondroitin 6-O-sulfotransferases (C6STs), which appear to have independently diverged in the ascidian lineage during evolution. Among them, Ci-C6ST-like1 and Ci-C6ST-like7 were predominantly expressed in the developing notochord. In addition, they were weakly expressed in the neural tube. The disruption of either one of them affected the convergent extension movement of notochordal cells. Presumptive notochord cells coming from both sides of the embryo did not intercalate. The results suggest that both of them are necessary. In some cases, the anterior neural tube failed to close. Forced expression of Ci-C6ST-like1 or Ci-C6ST-liRESULTS:ke7 in the notochord restored the normal intercalation of notochordal cells, indicating that the effects of morpholino oligos are specific.CONCLUSIONS:Ci-C6ST-like1 and Ci-C6ST-like7 are required for the morphogenesis of the notochord in the ascidian embryo.
DOI	10.1002/dvdy.24213
文献番号	25298188