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クラモト ゴロウ
藏本 吾郎 所属 研究施設 研究施設 職種 非常勤講師 |
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| 論文種別 | 原著 |
| 言語種別 | 英語 |
| 査読の有無 | 査読あり |
| 表題 | In-Vitro Decidualization With Different Progesterone Concentration: Development of a Hormone-Responsive 3D Endometrial Tissue Using Rat Endometrial Tissues. |
| 掲載誌名 | 正式名：Cureus 略 称：Cureus ISSNコード：21688184/21688184 |
| 掲載区分 | 国外 |
| 巻・号・頁 | 15(11),pp.e49613 |
| 著者・共著者 | TOMA Chihiro†*, KURAMOTO Goro, HOMMA Jun*, SAKAGUCHI Katsuhisa*, SHIMIZU Tatsuya |
| 担当区分 | 2nd著者 |
| 発行年月 | 2023/11/28 |
| 概要 | Infertility in women is associated with various uterine and ovarian disorders. Treatment strategies for infertility can range from medications to embryo implantation through assisted reproductive technology (ART). ART has enabled considerable progress; however, there is currently no treatment to replace the endometrium itself. Decidualization requires a complex interaction between endometrial tissue and estrogen and progesterone. We aimed to create a three-dimensional endometrial-like tissue model using in-vitro cell sheet engineering with rat endometrium, and culture cells at different progesterone concentrations to mimic local concentrations. Histological and morphological changes revealed that development of the endometrial-like tissue was not proportional to progesterone concentrations in terms of thickness, number of endometrial glands, or area fraction of intimal glands. These results suggest that decidualization may not be commensurate with the local endometrial progesterone concentration. Notably, the number of endometrial glands increased in the high concentration group and compaction occurred, indicating that the endometrial conditions in the high concentration group may be most conducive to increase pregnancy rates. These findings suggest that there may be an "optimal progesterone concentration" for decidualization, application of which may lead to new strategies for improving pregnancy rates in women with infertility. |
| DOI | 10.7759/cureus.49613 |
| PMID | 38033443 |