ミヤヤマ　タカミツ   MIYAYAMA Takamitsu   宮山　貴光    所属   医学部 医学科    職種   助教
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Separation of metallothionein isoforms extracted from isoform-specific knockdown cells on two-dimensional micro high-performance liquid chromatography hyphenated with inductively coupled plasma-mass spectrometry
掲載誌名	正式名：Journal of Analytical Atomic Spectrometry 略　 称：J Anal Atom Spectrom ISSNコード：0267-9477
巻・号・頁	22(2),pp.179-182
著者・共著者	MIYAYAMA Takamitsu†, OGRA Yasumitsu*, SUZUKI Kazuo
担当区分	筆頭著者
発行年月	2007
概要	A two-dimensional (2D) micro HPLC-inductively coupled plasma mass spectrometry (ICP-MS) system consisting of a gel filtration column and an anion-exchange column was constructed to separate two metallothionein (MT) isoforms. MT-I and MT-II genes were specifically knocked down with the RNA interference (RNAi) technique, and the effects of the knockdown were evaluated by the reverse transcription-polymerase chain reaction (RT-PCR) and the 2D micro HPLC-ICP-MS system. The minimum cell number required by our 2D micro HPLC-ICP-MS system was calculated to be 2.0 x 10(3), and the detection limit of MT protein was estimated to be 22.2 mg L-1.
DOI	Doi 10.1039/B613662c
文献番号	ISI:000243976900012